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Kreon Medical Systems San. and Tic. A.S.
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Adres: Uzayçağı Cad. 1155. Sk. No:21 Ostim Yenimahalle / Ankara

Tel 1: 0 (312) 354 9365

Tel 2: 0 (312) 354 9366

Faks:

Email 1: info@kreonmedikal.com.tr

Web: http://www.kreonmedikal.com.tr/

2.5 HEXANDIONE (Urine)

2.5 Hexandıone (Urine)

2.5 HEXANDIONE (Urine)

2,5-hexandione (Urine) For HPLC

 
INTENDED USE
KR149 Reagent kit for the HPLC determination of 2.5 -hexanedione in urine.
 
 
KIT SPECIFICATION
Contents for 100 analyzes
 

cat no

Quantity

Reagents

KR1491

1000mlx1

mobile phase

KR1492

100mlx1

Internal Standard

KR1493

0.5mlx5

Urine Calibrator

KR1494

5.0mlx4

Derivatization solution

KR1495

2.5mlx1

Hydrolysis Reagent

 

ACCESSORIES

 

KR1490

1 pc

HPLC column for the analysis of 2,5 -hexanedione in urine.

KR1499

100

Autosampler vial with teflon caps

 

CONTROLS 

KR1497

0.5ml x5

Urine Control, Level I

KR1498

0.5ml x5

Urine Control, Level II

 
INTRODUCTION
2.5 Hexandione is composed of an aliphatic diketone. The product of 2,5 Hexandione is transformation of the hexane. Glues which is used in the production of shoes or leathers contains hexane. Major way of absorption, even if ingestion is inhalation. Eye and skin contact can also happen. It can be metabolized so, 2.5 hexandione determination is decisive to determine the risk owing to the exposition to hexane.
From a clinical point of view 2,5 hexandione is considered one of the most significant metabolites of the hexane in the improvement of polyneuropathies in leather and shoemakers.
 
CHEMICAL ANALYSIS BY HPLC
2.5 Hexandione (PM=114.14) is an aliphatic volatile diketone. Because of its molecular structure, it is not directly revealed by UV determination.
This method allows to perform the HPLC urinary determination of 2,5 hexandione avoiding the extraction and concentration steps.
After acid hydrolysis, 2.5 hexandione is pulled with a certain reagent for ketones and then directly injected into the HPLC.
EQUIPMENT AND INSTRUMENTAL PARAMETERS
The analysis of 2,5 -hexandione requires a HPLC system including isocratic pump, autosampler and UV detector.
The use of a solvent degasser prevents air bubbles and ensure a stable baseline.
Sampler: Injection volume 50 μl, analytical run time 20 min..
HPLC pump: Flow rate 1.2 ml/min is recommended
Column temperature: Ambient (~25 °C)
UV detector: 334 nm

COLLECTION AND STORAGE OF PATIENT SAMPLES

Before and soon after work shift, collect 10 ml of urine in a suitable test tube.

The sample is stable up to 4 weeks at - 20 °C.

 

SAMPLE PREPARATION PROCEDURE

200 µl urine sample is added to vial with teflon cap

20 µl hydrolysis reagent is added.

Incubation is carried out at 100°C for 60 min.

Vortex the mix for 10s and then Centrifuge at 4 000 rpm for 5 min.

200 µl hydrolysis reagent is added

10 µl internal standard is added.

Incubation is carried

Samples prepared as above can be stored at ambient temperature for about 24 hours, at +2 to +8 °C up to 36 h, frozen below -18 °C up to 3 months.

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